Simoa™ Monthly Maintenance

Monthly Maintenance Step 1

Primary and Secondary Container Cleaning

18. Shutdown Software

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01.01 — Overview

This guide provides instructions for performing monthly user maintenance on the Simoa™ HD-1 Analyzer.

For information on using the Analyzer, see the Simoa HD-1 Analyzer User Guide (USER-104-01).

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01.02 — System Fluid Container Cleaning and Tubing Rinse

Once a month, the fluid resource containers must be cleaned. The tubing from the containers into the system must be primed with water for 30 minutes before they are primed with buffer for another 30 minutes.

During this procedure, discard all fluids and rinsates according to your company’s waste disposal policies.

The two types of fluid containers are defined as follows:

  • Secondary Container – This is the removable container with a screw cap where new fluid is added. The wash buffer 1 and DI Water secondary containers are identical in size, whereas the wash buffer 2 container is smaller.
  • Primary Container – This is the pyramidal-shaped container behind the secondary containers. The liquid level sensor and tubing are connected through a screw cap on top.

The tubing leading to the sensor cap is color coded as follows:

  • Blue — DI water
  • White — Wash buffer 1
  • Yellow — Wash buffer 2

Secondary Container and Primary Container Cleaning

  1. Close the Simoa HD-1 Analyzer software and shut down the instrument.
  2. Remove all secondary containers.
    • DI water and wash buffer 1 – Lift the container up and then pull out.
    • Wash buffer 2 – Pull the spring-loaded handle and then lift the container up.
  3. Empty and dispose of all secondary container fluids.
  4. Remove all primary containers. The process is the same for each fluid type.
    • Disconnect the sensor by pulling the black connector up.
    • Unscrew tubing
      Disconnect sensor.
    • Unscrew the tubing leading into the black cap.
    • Remove the primary container by pulling forward and then up.
    • Unscrew the black cap and place on a clean lint-free paper towel.
  5. Empty and dispose of all primary container fluids.
  6. Wash all primary and secondary containers by triple-rinsing them with DI water. Fill the containers to approximately 1/3 of their total capacity with DI water and shake to rinse all surfaces.
  7. Clean the liquid level sensors by running under DI water.

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Monthly Maintenance Step 2

Priming the System with DI Water

18. Shutdown Software

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02.01 — Priming the System with DI Water

  1. Fill the primary containers with DI water as follows. Do not fill the secondary containers.
    • DI Water — Full
    • Wash buffer 1 — Full
    • Wash buffer 2 — Half full
  2. Replace the sensor caps. If they have been separated from the containers, identify the caps by the physical differences in the tubing. Wash buffer 1 and wash buffer 2 use different connectors; the tubing will not connect to the system if the sensor caps are swapped. DI water uses blue tubing.
  3. Replace the primary containers into the instrument. Do not replace the secondary containers.
  4. Reconnect the sensor cable and screw the fluid tubing together. Refer to the color code listed previously to ensure proper container connections.
    • Twist the tubing approximately one turn to begin 'self-threading'; this ensures the tubing will not loosen. The sensor connection will make an audible click sound when done properly.
    • If the sensor is not connecting, check that the tabs on the sensor are properly aligned with the housing.
  5. Turn on the instrument and open the Simoa HD-1 software.
  6. After initialization completes, run the Monthly Fluid Prime task. This step takes approximately 30 minutes to complete.
    1. Touch the Maintenance Tab > touch the Monthly Fluid Prime task checkbox.
    2. Touch Run Task. The task takes approximately 30 minutes to complete. The task window has a timer that indicates when the task will complete.
  7. Close the Simoa HD-1 software and shut down the instrument.

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Monthly Maintenance Step 3

Priming the System with Wash Buffer

18. Shutdown Software

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03.01 — Priming the System with Wash Buffer

  1. Disconnect and remove the Wash Buffer 1 and Wash Buffer 2 primary containers. Do not remove the DI Water container.
  2. Unscrew the black caps and place the caps and the liquid level sensors on a clean lint-free paper towel.
  3. Empty the remaining water out of the containers.
  4. Replace the primary containers while they are still empty. Screw the sensor cap back on and connect the tubing and sensor again. Refer to the earlier instructions for detail.
  5. Fill the secondary containers with the following volumes:
    • DI Water – at least 5 L
    • Wash buffer 1 – at least 5 L
    • Wash buffer 2 – at least 2 L
  6. Replace the secondary containers into the instrument.
  7. Turn on the instrument and then open the Simoa HD-1 software.
  8. After initialization completes, run the Monthly Fluid Prime task again. This will take another 30 minutes to complete.

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Monthly Maintenance Step 4

Surface Cleaning

18. Shutdown Software

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04.01 — Cleaning the Touchscreen

Use any glass cleaner that does not contain ammonia.

Wet a paper towel with glass cleaner and then gently wipe down touchscreen. Do not spray glass cleaner directly onto the touchscreen.

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04.02 — Cleaning Exterior Surfaces

Clean exterior instrument surfaces by wiping with a soft, clean cloth moistened with water or 10% ethanol.

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04.03 — Other Surface Cleaning

  1. System Resource Drawers
    • Fully open both tip drawers from the instrument.
    • Wipe the metal frame beneath the drawers with water and then with 70% ethanol
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  2. Sample and Reagent Bays
    • Turn off the instrument.
    • Remove all reagent racks, plate racks, tube racks, and RGP racks. Wipe with water first and then with 70% ethanol.
    • Replace the racks.
  3. Bottom Cabinet
    • Wipe the surface beneath the system fluid containers with water and then 70% ethanol.
    • Wipe the surface beneath the fluid waste containers with water and then 70% ethanol.
    • Wipe the solid waste ramp with water and then 70% ethanol.

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04.04 — Completion

The system is now ready for use. All normal run maintenance must be performed before the next run (including the Start of Day task). Note the date of completion for record keeping and scheduling the next monthly maintenance procedure.

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